2FM presenter Jim-Jim Nugent announced he was quitting the station on a social networking site hours before RTE launches a new radio schedule.
The news came as it emerged that another radio host, Nikki Hayes, would be axed from 2fm.
Jim-Jim posted a YouTube video to his Facebook page yesterday featuring a cartoon version of CNN presenter Larry King publicising Jim-Jim's departure.
The animated character said: "It is with great sadness that I must tell you that Jim-Jim Nugent has resigned from RTE and 2fm today.
"There are many reasons but the main one was they refused to remove music of Glee from the fecking playlist," it claimed.
The curious announcement was well received by former work colleagues, according to the former host.
"Everyone had a good laugh at the video, I even got a text from programme director John McMahon, who said it was very funny," Jim-Jim said.
The 38-year-old, who had been co-presenting the Breakfast Show with Colm Hayes, said his goodbyes to loyal listeners on Friday and although he was offered other slots by RTE, he refused them.
"RTE has made me a number of offers, but I think now is the right time to do my own thing," he said.
He denied claims he was leaving because of an alleged rift with his colleague.
"Myself and Colm will remain great friends," he said.
"It was tough to walk away but that was only because Claire Duignan was holding his legs, crying and screaming."
The Larry King character says on the video that RTE was like the opposite of the Mafia -- in that they made Jim-Jim "an offer he couldn't accept".
Both comedian Hector O hEochagain and TV presenter Lucy Kennedy have been tipped for the morning slot.
It has also emerged that Nikki Hayes, who presented Strawberry Alarm Clock with Nugent before she moved to RTE, has been axed.
An insider revealed that the 31-year-old was "particularly upset" as she was taken off air before she could say her goodbyes to fans.
She was seen being embraced by colleagues on the steps of the radio building.