It faced stiff competition as Mrs Brown went head to head with Brit classic Absolutely Fabulous, Benidorm and The Big Bang Theory.
But 57-year-old Brendan took home the award which was voted for by the British public.
The Finglas man took to the O2 stage, in a polka-dot waistcoat and candy pink bow tie alongside members of the cast.
Many of Brendan's family play characters in the series and he joked: "The only person that's not in the show is my mother and that's because she is dead."
Mrs Brown's Boys has been a smash hit with viewers and continues to pull in incredible ratings. The comedy's Christmas special pulled in over 1.8m Irish viewers over two nights on RTE and over 17m when it was screened on the BBC.
But viewers may be disheartened to hear that a fourth series of Mrs Brown has been shelved as Brendan concentrates on developing a movie of the show.
The €4.5m movie, which is currently under the working title Mrs Brown: De Filum, will begin filming in August.
He also recently filmed a pilot for a new TV game show for the BBC, Mrs Brown's Celebrities. "Basically, it's Mrs Brown taking the mickey out of famous people because she doesn't know who they are," he said.
"She doesn't have any concept of celebrity. We shot a pilot episode with Kate Garraway, Robbie Savage and Russell Grant and they were crying with laughter."