Dundee-based publisher of the Dandy, DC Thomson confirmed earlier this year that tomorrow's edition of the comic will be the last before the title's much-loved characters go digital.
It follows a drop in circulation in recent years but the publishers insist the show will go on.
Ellis Watson, chief executive of the company's newspaper and magazine publishing operations, said: "Dan has certainly not eaten his last cow pie."
The announcement that this week's edition will be the last in print will likely see copies fly off the shelves as collectors vie for a piece of history.
During its peak circulation in the 1950s, the Dandy sold two million copies each week.This figure has dropped in recent years to around 8,000.
Its popular characters have included Bananaman and Beryl the Peril.